rabbit polyclonal antibody targeting rage  (Proteintech)

Journal: STAR Protocols

Article Title: Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

doi: 10.1016/j.xpro.2024.103484

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Article Snippet: Rabbit anti-mouse RAGE (1:100 dilution) , Boster , Cat# A03438; RRID: AB_3081636.

Techniques: Recombinant, Modification, Electron Microscopy, Software, Sterility

Journal: Burns & Trauma

Article Title: Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds

doi: 10.1093/burnst/tkac001

Figure Lengend Snippet: Cur suppresses AGEs/AGER axis-mediated ROS and ADSC apoptosis. ( a ) ADSCs pre-treated with AGEs were mixed with Cur or the p65 inhibitor PDTC (30 μM). Left: western blot demonstrates protein expression levels of AGER and phosphorylated and total p65. Right: comparison of AGER and p-p65 expression levels between the study groups. ( b ) ROS level was tested by fluorescence microscopy and total cell number was observed by bright field microscopy to calculate the percentage. ( c ) Flow cytometry showing ROS levels in ADSCs. ( d ) FITC/PI stained ADSCs analyzed by flow cytometry. Data are shown as means ± SD. Statistical analysis: * p <0.05, * * p <0.01 and * p <0.001. AGE Advanced glycation end product, AGER AGE receptor, ROS reactive oxygen species, ADSC adipose-derived stem cell, Cur curcumin, PDTC pyrrolidinedithiocarbamate

Article Snippet: The protein was transferred to a PVDF membrane and the primary antibody was incubated at 4°C overnight, followed by incubation with the secondary antibody (BA1054, Boster, Wuhan, China) at room temperature for 2 h. The primary antibodies were rabbit AGER (#55222, 1:500; Cell Signaling Technology/CST, Beverly, MA, USA), p65 (#3033, 1:500; CST), p-p65 (#8242, 1:500; CST) and β-actin (ab8227, 1:1000; Abcam, Cambridge, UK).

Techniques: Western Blot, Expressing, Comparison, Fluorescence, Microscopy, Flow Cytometry, Staining, Derivative Assay

Journal: Burns & Trauma

Article Title: Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds

doi: 10.1093/burnst/tkac001

Figure Lengend Snippet: Role of NF-κB p65 signal in the inhibitory effect of Cur on ADSCs. ( a ) Left: time course analysis of AGER and phosphorylated and total p65 protein expression levels in ADSCs pre-treated with AGEs (800 μg/mL; 24 h) treated with Cur (20 μM). Right: comparison of AGER and p-p65 expression levels between the study groups. ( b) ADSCs pre-treated with AGEs (800 μg/mL; 24 h) with or without Cur (20 μM; 24 h). Translocation of p65 from the cytoplasm to the nucleus was quantified by cell immunofluorescence staining (red arrowheads). ( c ) V-mCherry/caspase-3 stained ADSCs analyzed by flow cytometry. Relative fluorescence intensity was used to calculate cell apoptosis and caspase-3 expression level. Data are shown as means ± SD. Statistical analysis: * p <0.05, * * p <0.01 and * p <0.001. ( * p vs 0 h of AGER, # p vs 0 h of p-p65). NF-κB nuclear factor-κB, Cur curcumin, ADSC adipose-derived stem cell, AGE advanced glycation end product, AGER AGE receptor

Article Snippet: The protein was transferred to a PVDF membrane and the primary antibody was incubated at 4°C overnight, followed by incubation with the secondary antibody (BA1054, Boster, Wuhan, China) at room temperature for 2 h. The primary antibodies were rabbit AGER (#55222, 1:500; Cell Signaling Technology/CST, Beverly, MA, USA), p65 (#3033, 1:500; CST), p-p65 (#8242, 1:500; CST) and β-actin (ab8227, 1:1000; Abcam, Cambridge, UK).

Techniques: Expressing, Comparison, Translocation Assay, Immunofluorescence, Staining, Flow Cytometry, Fluorescence, Derivative Assay